Recombinant ferritin: modulation of subunit stoichiometry in bacterial expression systems

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Recombinant ferritin: modulation of subunit stoichiometry in bacterial expression systems.

We describe a strategy for the creation of recombinant ferritin heteropolymers which mimic the natural heterogeneity of this protein. This method entailed the co-expression of cDNA for both ferritin H and ferritin L subunits in a single bacterium using either a bicistronic vector, in which both cDNAs were expressed from the vector, or a dual vector expression strategy, in which each subunit was...

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ژورنال

عنوان ژورنال: Protein Engineering Design and Selection

سال: 1997

ISSN: 1741-0126,1741-0134

DOI: 10.1093/protein/10.8.967